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fusobacterium nucleatum atcc 10953  (ATCC)


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    Structured Review

    ATCC fusobacterium nucleatum atcc 10953
    Fusobacterium Nucleatum Atcc 10953, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 176 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atcc+10953/Fusobacterium+nucleatum%3B+subsp%2E+polymorphum/pm41820945-87-5-7
    Average 96 stars, based on 176 article reviews
    fusobacterium nucleatum atcc 10953 - by Bioz Stars, 2026-10
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    Polymerase Chain Reaction:

    Article Title: The gene encoding ornithine decarboxylase for putrescine biosynthesis is essential for the viability of Fusobacterium nucleatum .
    Article Snippet: .. Int, integrated strain representing an oda/Δoda merodiploid. (D) In the presence of pCWU6a-oda, which provides an ectopic copy of oda under its native promoter, viable Δoda mutants were successfully recovered following HicA-based counterselection, as confirmed by PCR. (E) Schematic of the CRISPRi system targeting oda, consisting of a theophylline-inducible dCas9 and a constitutively expressed sgRNA. (F) The specific 20-nt sequence within oda targeted by the sgRNA is shown (red line, position numbers indicated). (G) Growth of strain WTpZP4C(oda) was completely arrested upon theophylline induction (0.2–2.0 mM). (H) CRISPRi silencing of oda caused growth defects across multiple F. nucleatum subspecies, including CTI-2 (subsp. nucleatum), ATCC 51190 (subsp. vincentii), and ATCC 10953 (subsp. polymorphum). ..

    Sequencing:

    Article Title: The gene encoding ornithine decarboxylase for putrescine biosynthesis is essential for the viability of Fusobacterium nucleatum .
    Article Snippet: .. Int, integrated strain representing an oda/Δoda merodiploid. (D) In the presence of pCWU6a-oda, which provides an ectopic copy of oda under its native promoter, viable Δoda mutants were successfully recovered following HicA-based counterselection, as confirmed by PCR. (E) Schematic of the CRISPRi system targeting oda, consisting of a theophylline-inducible dCas9 and a constitutively expressed sgRNA. (F) The specific 20-nt sequence within oda targeted by the sgRNA is shown (red line, position numbers indicated). (G) Growth of strain WTpZP4C(oda) was completely arrested upon theophylline induction (0.2–2.0 mM). (H) CRISPRi silencing of oda caused growth defects across multiple F. nucleatum subspecies, including CTI-2 (subsp. nucleatum), ATCC 51190 (subsp. vincentii), and ATCC 10953 (subsp. polymorphum). ..

    Knock-Out:

    Article Title: Fusobacterium nucleatum manipulates host autophagy to promote its intracellular survival and treatment resistance in nasopharyngeal carcinoma.
    Article Snippet: .. Bacterial strain and culture conditions F. nucleatum strains, except for ATCC 10953 and its isogenic knockout strain F. nucleatum △FadA, were isolated, cultured, and purified from NPC tissues. ..

    Isolation:

    Article Title: Fusobacterium nucleatum manipulates host autophagy to promote its intracellular survival and treatment resistance in nasopharyngeal carcinoma.
    Article Snippet: .. Bacterial strain and culture conditions F. nucleatum strains, except for ATCC 10953 and its isogenic knockout strain F. nucleatum △FadA, were isolated, cultured, and purified from NPC tissues. ..

    Cell Culture:

    Article Title: Fusobacterium nucleatum manipulates host autophagy to promote its intracellular survival and treatment resistance in nasopharyngeal carcinoma.
    Article Snippet: .. Bacterial strain and culture conditions F. nucleatum strains, except for ATCC 10953 and its isogenic knockout strain F. nucleatum △FadA, were isolated, cultured, and purified from NPC tissues. ..

    Purification:

    Article Title: Fusobacterium nucleatum manipulates host autophagy to promote its intracellular survival and treatment resistance in nasopharyngeal carcinoma.
    Article Snippet: .. Bacterial strain and culture conditions F. nucleatum strains, except for ATCC 10953 and its isogenic knockout strain F. nucleatum △FadA, were isolated, cultured, and purified from NPC tissues. ..



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    Fusobacterium Nucleatum Atcc 10953, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Phylogenetic relationship of representative F. nucleatum strains used in this study, grouped by subspecies—subsp. nucleatum (FNN; ATCC 25586, ATCC 23726, CTI-2), subsp. vincentii (FNV; 3_1_27, ATCC 49256, ATCC 51190), subsp. animalis (FNA; 7_1, F0401, ATCC 51191), and subsp. polymorphum (FNP; ATCC 10953, 12230). The phylogenetic tree was constructed based on znpA gene using the maximum-likelihood method implemented in DNAMAN Version 10 (Lynnon Biosoft). Fusobacterium periodonticum ATCC 33693 (FP) was included as an outgroup. (B) Schematic of the chromosomal region between uraA and pepF showing subspecies-specific presence of luxS . luxS is absent from FNN and FNV at this locus, present as an intact gene in FNA (between uraA and pepF ), and disrupted in FNP by insertion of an IS200-family element. The corresponding region from F. periodonticum is shown for comparison. Arrows indicate gene orientation; uraA (gray), pepF (black), luxS (blue), IS200 insertion (magenta), and the adjacent gene ( ddpA , orange) are indicated. (C) AI-2 activity in cell-free culture supernatants was measured using the Vibrio harveyi BB170 bioluminescence reporter assay. Supernatants from FNN, FNV, and FNP strains showed signals at or near background levels, whereas all tested FNA strains and F. periodonticum generated robust reporter induction. E. coli wild type (WT) and its Δ luxS mutant served as positive and negative controls, respectively. Data are presented as relative fluorescence units (RFU; mean ± SD) from three independent experiments (each assayed in technical triplicate); the y-axis includes a break to display both low- and high-signal samples.
    Atcc 10953, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Phylogenetic relationship of representative F. nucleatum strains used in this study, grouped by subspecies—subsp. nucleatum (FNN; ATCC 25586, ATCC 23726, CTI-2), subsp. vincentii (FNV; 3_1_27, ATCC 49256, ATCC 51190), subsp. animalis (FNA; 7_1, F0401, ATCC 51191), and subsp. polymorphum (FNP; ATCC 10953, 12230). The phylogenetic tree was constructed based on znpA gene using the maximum-likelihood method implemented in DNAMAN Version 10 (Lynnon Biosoft). Fusobacterium periodonticum ATCC 33693 (FP) was included as an outgroup. (B) Schematic of the chromosomal region between uraA and pepF showing subspecies-specific presence of luxS . luxS is absent from FNN and FNV at this locus, present as an intact gene in FNA (between uraA and pepF ), and disrupted in FNP by insertion of an IS200-family element. The corresponding region from F. periodonticum is shown for comparison. Arrows indicate gene orientation; uraA (gray), pepF (black), luxS (blue), IS200 insertion (magenta), and the adjacent gene ( ddpA , orange) are indicated. (C) AI-2 activity in cell-free culture supernatants was measured using the Vibrio harveyi BB170 bioluminescence reporter assay. Supernatants from FNN, FNV, and FNP strains showed signals at or near background levels, whereas all tested FNA strains and F. periodonticum generated robust reporter induction. E. coli wild type (WT) and its Δ luxS mutant served as positive and negative controls, respectively. Data are presented as relative fluorescence units (RFU; mean ± SD) from three independent experiments (each assayed in technical triplicate); the y-axis includes a break to display both low- and high-signal samples.
    Fpo Atcc, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Phylogenetic relationship of representative F. nucleatum strains used in this study, grouped by subspecies—subsp. nucleatum (FNN; ATCC 25586, ATCC 23726, CTI-2), subsp. vincentii (FNV; 3_1_27, ATCC 49256, ATCC 51190), subsp. animalis (FNA; 7_1, F0401, ATCC 51191), and subsp. polymorphum (FNP; ATCC 10953, 12230). The phylogenetic tree was constructed based on znpA gene using the maximum-likelihood method implemented in DNAMAN Version 10 (Lynnon Biosoft). Fusobacterium periodonticum ATCC 33693 (FP) was included as an outgroup. (B) Schematic of the chromosomal region between uraA and pepF showing subspecies-specific presence of luxS . luxS is absent from FNN and FNV at this locus, present as an intact gene in FNA (between uraA and pepF ), and disrupted in FNP by insertion of an IS200-family element. The corresponding region from F. periodonticum is shown for comparison. Arrows indicate gene orientation; uraA (gray), pepF (black), luxS (blue), IS200 insertion (magenta), and the adjacent gene ( ddpA , orange) are indicated. (C) AI-2 activity in cell-free culture supernatants was measured using the Vibrio harveyi BB170 bioluminescence reporter assay. Supernatants from FNN, FNV, and FNP strains showed signals at or near background levels, whereas all tested FNA strains and F. periodonticum generated robust reporter induction. E. coli wild type (WT) and its Δ luxS mutant served as positive and negative controls, respectively. Data are presented as relative fluorescence units (RFU; mean ± SD) from three independent experiments (each assayed in technical triplicate); the y-axis includes a break to display both low- and high-signal samples.
    Fpo Atcc 10953 Fnuc, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    (A) Phylogenetic relationship of representative F. nucleatum strains used in this study, grouped by subspecies—subsp. nucleatum (FNN; ATCC 25586, ATCC 23726, CTI-2), subsp. vincentii (FNV; 3_1_27, ATCC 49256, ATCC 51190), subsp. animalis (FNA; 7_1, F0401, ATCC 51191), and subsp. polymorphum (FNP; ATCC 10953, 12230). The phylogenetic tree was constructed based on znpA gene using the maximum-likelihood method implemented in DNAMAN Version 10 (Lynnon Biosoft). Fusobacterium periodonticum ATCC 33693 (FP) was included as an outgroup. (B) Schematic of the chromosomal region between uraA and pepF showing subspecies-specific presence of luxS . luxS is absent from FNN and FNV at this locus, present as an intact gene in FNA (between uraA and pepF ), and disrupted in FNP by insertion of an IS200-family element. The corresponding region from F. periodonticum is shown for comparison. Arrows indicate gene orientation; uraA (gray), pepF (black), luxS (blue), IS200 insertion (magenta), and the adjacent gene ( ddpA , orange) are indicated. (C) AI-2 activity in cell-free culture supernatants was measured using the Vibrio harveyi BB170 bioluminescence reporter assay. Supernatants from FNN, FNV, and FNP strains showed signals at or near background levels, whereas all tested FNA strains and F. periodonticum generated robust reporter induction. E. coli wild type (WT) and its Δ luxS mutant served as positive and negative controls, respectively. Data are presented as relative fluorescence units (RFU; mean ± SD) from three independent experiments (each assayed in technical triplicate); the y-axis includes a break to display both low- and high-signal samples.
    Fpo Atcc 10953, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atcc+10953/Fusobacterium+nucleatum%3B+subsp%2E+polymorphum/pm41656911-527-9-10
    Average 96 stars, based on 1 article reviews
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    strains atcc 10953  (ATCC)
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    (A) Phylogenetic relationship of representative F. nucleatum strains used in this study, grouped by subspecies—subsp. nucleatum (FNN; ATCC 25586, ATCC 23726, CTI-2), subsp. vincentii (FNV; 3_1_27, ATCC 49256, ATCC 51190), subsp. animalis (FNA; 7_1, F0401, ATCC 51191), and subsp. polymorphum (FNP; ATCC 10953, 12230). The phylogenetic tree was constructed based on znpA gene using the maximum-likelihood method implemented in DNAMAN Version 10 (Lynnon Biosoft). Fusobacterium periodonticum ATCC 33693 (FP) was included as an outgroup. (B) Schematic of the chromosomal region between uraA and pepF showing subspecies-specific presence of luxS . luxS is absent from FNN and FNV at this locus, present as an intact gene in FNA (between uraA and pepF ), and disrupted in FNP by insertion of an IS200-family element. The corresponding region from F. periodonticum is shown for comparison. Arrows indicate gene orientation; uraA (gray), pepF (black), luxS (blue), IS200 insertion (magenta), and the adjacent gene ( ddpA , orange) are indicated. (C) AI-2 activity in cell-free culture supernatants was measured using the Vibrio harveyi BB170 bioluminescence reporter assay. Supernatants from FNN, FNV, and FNP strains showed signals at or near background levels, whereas all tested FNA strains and F. periodonticum generated robust reporter induction. E. coli wild type (WT) and its Δ luxS mutant served as positive and negative controls, respectively. Data are presented as relative fluorescence units (RFU; mean ± SD) from three independent experiments (each assayed in technical triplicate); the y-axis includes a break to display both low- and high-signal samples.
    Strains Atcc 10953, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atcc+10953/Fusobacterium+nucleatum%3B+subsp%2E+polymorphum/pm41656911-140-3-4
    Average 96 stars, based on 1 article reviews
    strains atcc 10953 - by Bioz Stars, 2026-10
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    strain fpo atcc 10953  (ATCC)
    96
    ATCC strain fpo atcc 10953
    (A) Phylogenetic relationship of representative F. nucleatum strains used in this study, grouped by subspecies—subsp. nucleatum (FNN; ATCC 25586, ATCC 23726, CTI-2), subsp. vincentii (FNV; 3_1_27, ATCC 49256, ATCC 51190), subsp. animalis (FNA; 7_1, F0401, ATCC 51191), and subsp. polymorphum (FNP; ATCC 10953, 12230). The phylogenetic tree was constructed based on znpA gene using the maximum-likelihood method implemented in DNAMAN Version 10 (Lynnon Biosoft). Fusobacterium periodonticum ATCC 33693 (FP) was included as an outgroup. (B) Schematic of the chromosomal region between uraA and pepF showing subspecies-specific presence of luxS . luxS is absent from FNN and FNV at this locus, present as an intact gene in FNA (between uraA and pepF ), and disrupted in FNP by insertion of an IS200-family element. The corresponding region from F. periodonticum is shown for comparison. Arrows indicate gene orientation; uraA (gray), pepF (black), luxS (blue), IS200 insertion (magenta), and the adjacent gene ( ddpA , orange) are indicated. (C) AI-2 activity in cell-free culture supernatants was measured using the Vibrio harveyi BB170 bioluminescence reporter assay. Supernatants from FNN, FNV, and FNP strains showed signals at or near background levels, whereas all tested FNA strains and F. periodonticum generated robust reporter induction. E. coli wild type (WT) and its Δ luxS mutant served as positive and negative controls, respectively. Data are presented as relative fluorescence units (RFU; mean ± SD) from three independent experiments (each assayed in technical triplicate); the y-axis includes a break to display both low- and high-signal samples.
    Strain Fpo Atcc 10953, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/atcc+10953/Fusobacterium+nucleatum%3B+subsp%2E+polymorphum/pm41656911-366-1-3
    Average 96 stars, based on 1 article reviews
    strain fpo atcc 10953 - by Bioz Stars, 2026-10
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    Image Search Results


    (A) Phylogenetic relationship of representative F. nucleatum strains used in this study, grouped by subspecies—subsp. nucleatum (FNN; ATCC 25586, ATCC 23726, CTI-2), subsp. vincentii (FNV; 3_1_27, ATCC 49256, ATCC 51190), subsp. animalis (FNA; 7_1, F0401, ATCC 51191), and subsp. polymorphum (FNP; ATCC 10953, 12230). The phylogenetic tree was constructed based on znpA gene using the maximum-likelihood method implemented in DNAMAN Version 10 (Lynnon Biosoft). Fusobacterium periodonticum ATCC 33693 (FP) was included as an outgroup. (B) Schematic of the chromosomal region between uraA and pepF showing subspecies-specific presence of luxS . luxS is absent from FNN and FNV at this locus, present as an intact gene in FNA (between uraA and pepF ), and disrupted in FNP by insertion of an IS200-family element. The corresponding region from F. periodonticum is shown for comparison. Arrows indicate gene orientation; uraA (gray), pepF (black), luxS (blue), IS200 insertion (magenta), and the adjacent gene ( ddpA , orange) are indicated. (C) AI-2 activity in cell-free culture supernatants was measured using the Vibrio harveyi BB170 bioluminescence reporter assay. Supernatants from FNN, FNV, and FNP strains showed signals at or near background levels, whereas all tested FNA strains and F. periodonticum generated robust reporter induction. E. coli wild type (WT) and its Δ luxS mutant served as positive and negative controls, respectively. Data are presented as relative fluorescence units (RFU; mean ± SD) from three independent experiments (each assayed in technical triplicate); the y-axis includes a break to display both low- and high-signal samples.

    Journal: bioRxiv

    Article Title: AI-2 Production in Fusobacterium nucleatum Is Subspecies-Specific and Uncoupled from Quorum Sensing

    doi: 10.64898/2026.03.02.709096

    Figure Lengend Snippet: (A) Phylogenetic relationship of representative F. nucleatum strains used in this study, grouped by subspecies—subsp. nucleatum (FNN; ATCC 25586, ATCC 23726, CTI-2), subsp. vincentii (FNV; 3_1_27, ATCC 49256, ATCC 51190), subsp. animalis (FNA; 7_1, F0401, ATCC 51191), and subsp. polymorphum (FNP; ATCC 10953, 12230). The phylogenetic tree was constructed based on znpA gene using the maximum-likelihood method implemented in DNAMAN Version 10 (Lynnon Biosoft). Fusobacterium periodonticum ATCC 33693 (FP) was included as an outgroup. (B) Schematic of the chromosomal region between uraA and pepF showing subspecies-specific presence of luxS . luxS is absent from FNN and FNV at this locus, present as an intact gene in FNA (between uraA and pepF ), and disrupted in FNP by insertion of an IS200-family element. The corresponding region from F. periodonticum is shown for comparison. Arrows indicate gene orientation; uraA (gray), pepF (black), luxS (blue), IS200 insertion (magenta), and the adjacent gene ( ddpA , orange) are indicated. (C) AI-2 activity in cell-free culture supernatants was measured using the Vibrio harveyi BB170 bioluminescence reporter assay. Supernatants from FNN, FNV, and FNP strains showed signals at or near background levels, whereas all tested FNA strains and F. periodonticum generated robust reporter induction. E. coli wild type (WT) and its Δ luxS mutant served as positive and negative controls, respectively. Data are presented as relative fluorescence units (RFU; mean ± SD) from three independent experiments (each assayed in technical triplicate); the y-axis includes a break to display both low- and high-signal samples.

    Article Snippet: In our experiments, the signal levels previously reported for ATCC 10953 were comparable to background readings( ).

    Techniques: Construct, Comparison, Activity Assay, Reporter Assay, Generated, Mutagenesis, Fluorescence